anti-nfix  (Thermo Fisher)

Journal: Journal of Molecular Medicine (Berlin, Germany)

Article Title: Nuclear factor IX promotes endometriosis progression through transcriptional activation of tetraspanin-2

doi: 10.1007/s00109-026-02665-x

Figure Lengend Snippet: Difference in TSPAN2 expression between EMs and ESCs. A Venny platform identified 33 intersecting targets of the NFIX target genes from KnockTF2.0 database ( GSE45492 , q < 0.1) and differentially expressed genes from our previous RNA sequencing database (q < 0.1). B Heatmap of the 33 intersecting targets. C The mRNA levels of TSPAN2 were measured using real-time PCR in paired EMs and ESCs ( n = 15, *** P < 0.001, t- test). D The protein levels of TSPAN2 in paired EMs and ESCs were analyzed with western blot ( n = 15, ** P < 0.01, t- test). E Immunohistochemical staining was performed with samples of paired eutopic endometrium and ectopic endometrium ( n = 6, original magnification was × 200). F ESCs were transfected with si-NFIX, and the expression levels of TSPAN2 were analyzed by real-time PCR and western blot ( n = 5; * P < 0.05, ** P < 0.01, t- test). G ESCs were transfected with pENTER-NFIX, and the expression levels of TSPAN2 were analyzed by real-time PCR and western blot ( n = 5; *** P < 0.001, t- test)

Article Snippet: Subsequently, the paraffin-coated sections were subjected to incubation using primary antibody against NFIX (1:500; Abclonal, A9390) and an antibody against TSPAN2 (1:200; Invitrogen, PA5-53355).

Techniques: Expressing, RNA Sequencing, Real-time Polymerase Chain Reaction, Western Blot, Immunohistochemical staining, Staining, Transfection

Journal: Journal of Molecular Medicine (Berlin, Germany)

Article Title: Nuclear factor IX promotes endometriosis progression through transcriptional activation of tetraspanin-2

doi: 10.1007/s00109-026-02665-x

Figure Lengend Snippet: TSPAN2 is involved in the modulation of ESCs proliferation and invasion by NFIX. A ESCs were harvested for western blot after transfection with si-TSPAN2. The knockdown efficiency was verified ( n = 3, ** P < 0.01; t- test). B The transfection efficiency of pENTER-TSPAN2 was verified by western blot ( n = 3, ** P < 0.01; t- test). C and D ESCs were transfected with the si-TSPAN2, then harvested for real-time RT-PCR and western blot ( n = 3, * P < 0.05; t- test). E and F ESCs were transfected with the pENTER-TSPAN2, then harvested for real-time RT-PCR and western blot ( n = 3, * P < 0.05, ** P < 0.01, *** P < 0.001, t- test). G The cell proliferation and invasion activities were detected by CCK8 and transwell invasion assay in ESCs with si-TSPAN2 transfection ( n = 3, * P < 0.05; t- test). H The cell proliferation and invasion activities were detected by CCK8 and transwell invasion assay in ESCs with pENTER-TSPAN2 transfection ( n = 3, * P < 0.05; t- test). I Real-time PCR and western blot were used to analyze the expression level of Cyclin B1, MMP9, NFIX and TSPAN2 in the co-transfected ESCs ( n = 3, * P < 0.05, ANOVA ). J The cell proliferation and invasion activities were measured in co-transfected ESCs ( n = 3, * P < 0.05, *** P < 0,001, **** P < 0.0001; ANOVA )

Article Snippet: Subsequently, the paraffin-coated sections were subjected to incubation using primary antibody against NFIX (1:500; Abclonal, A9390) and an antibody against TSPAN2 (1:200; Invitrogen, PA5-53355).

Techniques: Western Blot, Transfection, Knockdown, Quantitative RT-PCR, Transwell Invasion Assay, Real-time Polymerase Chain Reaction, Expressing

Journal: Journal of Molecular Medicine (Berlin, Germany)

Article Title: Nuclear factor IX promotes endometriosis progression through transcriptional activation of tetraspanin-2

doi: 10.1007/s00109-026-02665-x

Figure Lengend Snippet: Identification of the critical binding site of NFIX on the TSPAN2 promoter in regulation. A Schematic presentation of 5 potential binding sites of NFIX on the TSPAN2 promoter, −1406bp to −1398bp (P1), −1289bp to −1281bp (P2), −1255bp to −1247bp (P3), −771bp to −763bp (P4) and −408bp to −400bp (P5). B Full length as well as 4 kinds of 5′-delete constructs of TSPAN2 promoter fragments were cloned upstream to luciferase reporter gene. The reporter plasmids were transiently transfected into ECC-1, and luciferase activities were measured ( n = 3, * P < 0.05, ** P < 0.01, t- test). C ESCs and EMs were subjected to ChIP using anti-NFIX antibody or IgG to validate the NFIX binding region of TSPAN2 promoter by real-time PCR ( n = 3, * P < 0.05, t- test)

Article Snippet: Subsequently, the paraffin-coated sections were subjected to incubation using primary antibody against NFIX (1:500; Abclonal, A9390) and an antibody against TSPAN2 (1:200; Invitrogen, PA5-53355).

Techniques: Binding Assay, Construct, Clone Assay, Luciferase, Transfection, Real-time Polymerase Chain Reaction

Journal: Cell reports

Article Title: Molecular cascade reveals sequential milestones underlying hippocampal neural stem cell development into an adult state

doi: 10.1016/j.celrep.2024.114339

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit anti-Nfix , Thermo Fisher Scientific , Cat# PA5–30897; RRID: AB_2548371.

Techniques: Recombinant, Electron Microscopy, Fluorescence, Reverse Transcription, Plasmid Preparation, Flow Cytometry, Software